Journal: Thrombosis Journal

Article Title: Long non-coding RNA crnde promotes deep vein thrombosis by sequestering miR-181a-5p away from thrombogenic Pcyox1l

doi: 10.1186/s12959-023-00480-9

Figure Lengend Snippet: High-throughput transcriptome sequencing analysis to screen key genes involved in DVT. A, DVT in the sham-operated mice and DVT mice observed by HE staining. White triangles indicate the platelet bundles, red triangles indicate clustered red blood cells, and yellow arrows indicate the blood vessel wall. B, Representative image of thrombosis in the inferior vena cava of the sham-operated mice and DVT mice. C & D, Measurement results of the length (C) and weight (D) of thrombus in the sham-operated mice and DVT mice (n = 6). E-G, The levels of inflammatory factors IL-1β (E), IL-6 (F) and IL-8 (G) in mouse femoral venous blood of the sham-operated mice and DVT mice measured by ELISA (n = 6). H & I, Volcano plots of differentially expressed lncRNAs (H) and mRNAs (I) analyzed by high-throughput sequencing (Black dots represent genes not differentially expressed, red dots represent the upregulated genes, and green dots represent downregulated genes. n = 3). J, The Crnde expression in DVT mice measured by RT-qPCR (n = 6). *** p < 0.001 vs. the sham group

Article Snippet: Mouse serum levels of IL-1β, IL-6, and IL-8 were determined using the ELISA kit for IL-1β (ab100704, Abcam, Cambridge, UK), IL-6 (ab100712, Abcam), and IL-8 (SEKM-0046, Solarbio, Beijing, China), referring to the kit instructions [ ].

Techniques: High Throughput Screening Assay, Sequencing, Staining, Enzyme-linked Immunosorbent Assay, Next-Generation Sequencing, Expressing, Quantitative RT-PCR

Journal: Thrombosis Journal

Article Title: Long non-coding RNA crnde promotes deep vein thrombosis by sequestering miR-181a-5p away from thrombogenic Pcyox1l

doi: 10.1186/s12959-023-00480-9

Figure Lengend Snippet: Overexpression of Pcyox1l reverses the inhibitory effect of Crnde silencing on DVT in mice. A, The expression of Crnde, miR-181a-5p and Pcyox1l in DVT mice treated with sh-Crnde alone or combined with oe-Pcyox1l determined by RT-qPCR. B, Western blot for the Pcyox1l protein expression in DVT mice treated with sh-Crnde alone or combined with oe-Pcyox1l. C & D, Images (C) and quantitation (D) of Pcyox1l protein expression in the inferior vena cava tissue of the DVT mice treated with sh-Crnde alone or combined with oe-Pcyox1l determined by immunohistochemistry. E, Representative image of thrombosis in inferior vena cava of the DVT mice in response to Crnde knockdown alone or combined with Pcyox1l overexpression. F & G, Measurement results of thrombus length (F) and thrombus weight (G) of the DVT mice in response to Crnde knockdown alone or combined with Pcyox1l overexpression. H-J, The levels of inflammatory factors IL-1β (H), IL-6 (I) and IL-1 and IL-8 (J) in the femoral venous blood of the DVT mice in response to Crnde knockdown alone or combined with Pcyox1l overexpression measured by ELISA. K, HE staining showing vascular inflammatory injury and platelet bundles in the DVT mice in response to Crnde knockdown alone or combined with Pcyox1l overexpression. Red triangles indicate the clustered red blood cells, and yellow arrows indicate the blood vessel wall. *** p < 0.001 vs. the sh-NC + oe-NC group. ### p < 0.001 vs. the sh-Crnde + oe-NC group. n = 6

Article Snippet: Mouse serum levels of IL-1β, IL-6, and IL-8 were determined using the ELISA kit for IL-1β (ab100704, Abcam, Cambridge, UK), IL-6 (ab100712, Abcam), and IL-8 (SEKM-0046, Solarbio, Beijing, China), referring to the kit instructions [ ].

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Western Blot, Quantitation Assay, Immunohistochemistry, Knockdown, Enzyme-linked Immunosorbent Assay, Staining

Journal: Neurochemical Research

Article Title: Morin Improves Cognitive Deficits in an in Vivo Model of Vascular Dementia by Modulating the N-methyl-D-aspartate Receptor Signaling Pathways

doi: 10.1007/s11064-026-04717-7

Figure Lengend Snippet: Morin modulated the expression of NMDA receptors in the hippocampus of VaD rats. a the expression levels of NR1 ; b the expression levels of NR2A ; c the expression levels of NR2B ; d the expression levels of NR1 protein; e the expression levels of NR2A protein; f the expression levels of NR2B protein; g protein levels of p-CREB; h protein levels of p-CAMK2A; i protein levels of p-CAMK2D. Protein levels of NR1, NR2A, and NR2B were quantified by ELISA. Data are presented as mean ± SD ( n = 8 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post-hoc test (data met assumptions of normality and homoscedasticity)/Kruskal-Wallis with Dunn’s test (data did not meet assumptions). * indicates a significant difference from the Sham group; # indicates a significant difference 2VO group; *# indicates a significant difference from both the Sham and 2VO groups, with a p-value of less than 0.05 considered statistically significant

Article Snippet: The phosphorylation status of another key NMDAR downstream effector, CREB at Ser133 (p-CREB), was quantified in hippocampal homogenates using a commercial ELISA kit (Phospho-CREB [Ser133] ELISA Kit, Boster Bio, #EKC2382).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay